Lumit® IL-1β Human/Mouse Immunoassay
No-Wash Immunoassay for IL-1β Detection in Cells
- Fast add-and-read protocol performed directly on cells
- Luminescent detection with just a standard plate-reading luminometer
- Scalable to 96- or 384- well plates, protocols included
- Pack sizes show assay number for 96-well plates
Catalog Number:
Choose a product
Size
Catalog Number: W6010
Catalog Number: W6011
Catalog Number: W6012
Catalog Number: W7010
Catalog Number: W7011
Catalog Number: W7012
Catalog Number: W116A-C
Catalog Number: W119A-C
Quantificare l'attivazione dell'inflammasoma senza noiose fasi di lavaggio
Il Lumit™ IL-1β Immunoassay misura quantitativamente l'IL-1β rilasciata in campioni di colture cellulari utilizzando un protocollo semplice e senza lavaggio. È sufficiente aggiungere gli anticorpi marcati al campione, aggiungere il reagente di rilevazione e leggere il segnale luminescente utilizzando un luminometro standard a lettura di piastra. L'intero protocollo viene completato in meno di 70 minuti! Il test può essere utilizzato direttamente sulle cellule in coltura o sul terreno di coltura trasferito in una piastra di analisi separata.
Come funziona il Lumit™ IL-1β Immunoassay
Protocollo semplice a una piastra, senza fasi di lavaggio!
Ampia gamma dinamica di rilevamento Lumit™
L'intervallo lineare da picomolare a nanomolare del Lumit™ copre le concentrazioni previste per la maggior parte dei modelli cellulari.
| Test | IL-1β (umano) Immunoassay | IL-1β (Topo) Immunodosaggio |
| Limite di rilevamento (LOD) | 10pg/ml (3 SD sopra il fondo) | 8pg/ml (3 SD sopra il fondo) |
| Intervallo dinamico | 22pg/ml – 40ng/ml | 11pg/ml – 20ng/ml |
| Dose minima rilevabile (MDD) | 7pg/ml (2 SD sopra il fondo) | 5pg/ml (2 SD sopra il fondo) |
| Tempo di analisi | 70 minuti | |
| Tipo di campione | Surnatanti di colture cellulari | |
SD=Deviazione Standard
Screening ad alto rendimento di inibitori dell'inflammasoma NLRP3
L'inibitore dell'inflammasoma NLRP3, MCC950, impedisce l'attivazione della caspasi-1 e il rilascio di IL-1 maturaβ. Una titolazione di MCC950 è stata aggiunta a cellule THP-1 differenziate (5 ×104/pozzetto) seguite da LPS (20 EU/ml) o da un veicolo. L'immunodosaggio Lumit™ IL-1β (Human) è stato eseguito dopo 5 ore.
Featured Publication
This peer-reviewed study "Interrogating direct NLRP3 engagement and functional inflammasome inhibition using cellular assays" examines the direct engagement and functionality of NLRP3 pathway activity in cells using several mechanistic assays, including the Lumit® IL-1β Immunoassay and Caspase-Glo 1 Inflammasome Assay.
Esplora altri test di rilevamento delle citochine
Offriamo un portfolio of assays to detect cytokines crescente di campioni di colture cellulari.
Non riuscite a’trovare il vostro target nell'elenco?
Please enquire per l'accesso anticipato ai nuovi saggi in fase di sviluppo.
Per una biotecnologia che sa che il tempo ha un valore significativo, gli immunodosaggi Lumit™ sono un modo essenziale per raccogliere rapidamente dati affidabili in modo da poter giungere a conclusioni e prendere decisioni in tempi brevi.
Jonathan Chow, scienziato principale, Corner Therapeutics
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
Anti hIL-1β mAb-SmBiT, 1000X |
W117A | 1 × 15μl | |
Anti hIL-1β mAb-LgBiT, 1000X |
W118A | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
Anti hIL-1β mAb-SmBiT, 1000X |
W117B | 1 × 150μl | |
Anti hIL-1β mAb-LgBiT, 1000X |
W118B | 1 × 150μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
Anti hIL-1β mAb-SmBiT, 1000X |
W117A | 5 × 15μl | |
Anti hIL-1β mAb-LgBiT, 1000X |
W118A | 5 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 1 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 1 × 3.2ml | |
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
Anti mIL-1β mAb-SmBiT, 1000X |
W120A | 1 × 15μl | |
Anti mIL-1β mAb-LgBiT, 1000X |
W121A | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405B | 1 × 1.25ml | |
Lumit® Detection Buffer B |
VB406B | 1 × 25ml | |
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
Anti mIL-1β mAb-SmBiT, 1000X |
W120B | 1 × 150μl | |
Anti mIL-1β mAb-LgBiT, 1000X |
W121B | 1 × 150μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Lumit® Detection Substrate B |
VB405A | 5 × 160μl | |
Lumit® Detection Buffer B |
VB406A | 5 × 3.2ml | |
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
Anti mIL-1β mAb-SmBiT, 1000X |
W120A | 5 × 15μl | |
Anti mIL-1β mAb-LgBiT, 1000X |
W121A | 5 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Human IL-1β Standard |
W116A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
Mouse IL-1β Standard |
W119A | 1 × 25μl | 10μg/ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Please see the Protocol for detailed storage conditions.
Resources
Articles
- Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Application Note: Culturing THP-1 Cells for Inflammasome Activation Assays
- High-Throughput Cytokine Detection with Lumit® Technology
- A Comprehensive Toolkit for NLRP3 Inflammasome Drug Discovery
- Targeted zinc-finger repressors to the oncogenic HBZ gene inhibit adult T-cell leukemia (ATL) proliferation
- Structural basis for thioredoxin-mediated suppression of NLRP1 inflammasome
- ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis
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Frequently Used With
Test Caspasi-Glo® 1 Inflammasoma
Un metodo omogeneo e bioluminescente per misurare selettivamente l'attività della caspasi-1, un componente essenziale dell'inflammasoma.
G9951, G9952, G9953
Thaw-and-Use Primary Effector Cells
Consistent and robust method for target cell killing.
CS3055C02, CS3055C03, CS3055B03, CS3055B06, CS3055A15, CS3055A18
Lumit® Immunoassay Labeling Kit and Detection Reagents
Antibody labeling kit and detection reagents to help develop Lumit® Immunoassays.
VB2500, VB2010, VB2020, VB2030, VB4050, VB4060
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
