Notes: Polyadenylated RNA [poly(A)-RNA] was purified from 0.5 mg total RNA using the PolyATtract® mRNA Isolation System. For primer extension, a primer was radiolabeled by 5' phosphorylation with [γ-³²P]ATP using the Primer Extension System. The primer extension reaction was performed using 10 µg of total RNA from adult Canton-S flies and 100 fmol of a ³²P-labeled primer. Size markers were a ³²P-labeled Promega phiX174/Hinf I Marker standard. (0588)