Recombinant Clone Screening Using the GoTaq Hot Start Green Master Mix

Sarah Wheeler
Promega Corporation
Publication Date: 2009

Abstract

We describe using the new GoTaq® Hot Start Green Master Mix to screen recombinant clones from single colonies on a plate and from plasmid minipreps. The master mix formulation contains direct-to-gel loading components, which provide convenience and allow fast PCR setup. With the GoTaq® Hot Start Polymerase technology, reactions can be assembled and stored at room temperature for up to 24 hours prior to cycling.

Promega Notes 101, 13–14.