IL-23 Bioassay Technical Manual
Instructions for Use of Product(s)
JA2511, JA2515, JA3511, JA3515
Literature # TM609
The IL-23 Bioassay is a bioluminescent cell-based assay designed to measure IL-23 stimulation or inhibition. The IL-23 Bioassay Cells are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-23 Bioassay consists of human cells engineered to express the IL-23 receptor and a luciferase reporter driven by a response element (RE). When IL-23 binds to IL-23R, intracellular signals are transduced, resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 7/25 revision of this document:
1. Revised text about the label in Section 3.
2. Updated the fonts.
3. Made miscellaneous text edits, including removing one patent statement and updating another patent statement.
4. Updated Lumit trademark to registered.
Revised 7/25.