Glycogen-Glo™ Assay Technical Manual
Instructions for Use of Product(s)
J5051, J5052
Literature # TM714
The Glycogen-Glo™ Assay is a bioluminescent assay for the rapid and sensitive detection of glycogen in biological samples. Glycogen is a large intracellular polymer of glucose synthesized by cells as a means of storing glucose to supply energy needs at both the cellular and organism levels. The glucose can be quickly mobilized to meet cell energy demands without relying on external glucose sources. The liver has large reserves of glycogen, which are used to sustain glucose blood levels and maintain glucose homeostasis throughout the body.
The Glycogen-Glo™ Assay uses two sequential steps to measure glycogen. Glycogen is first digested into glucose units by Glucoamylase enzyme. Then glucose is measured by coupling glucose oxidation and NADH production with a bioluminescent NADH detection system.
Summary of Changes
The following changes have been made to the 7/24 revision of this document:
1. In Section 5.A, two Thermo Fisher Scientific catalog numbers were updated.
2. Third party trademarks were added and updated.
Revised 7/24.