Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates, Technical Manual
Instructions for Use of Product(s)
N7221: Anti-HiBiT Monoclonal Antibody, XFD647, N7231: Anti-HiBiT Monoclonal Antibody, XFD488
Literature # TM772
Anti-HiBiT Monoclonal Antibody is a potent and specific mouse monoclonal antibody (mAb) that binds to the HiBiT tag, enabling applications such as immunoblotting, immunofluorescence, immunoprecipitation and fluorescence-activated cell sorting (FACS). The antibody binds to HiBiT with high affinity, with a KD in solution of approximately 6pM. Because of this high affinity and low background binding, many HiBiT-tagged proteins can be detected at endogenous expression levels.
Anti-HiBiT Monoclonal Antibody, XFD Fluorophore Conjugates, are fluorescently labeled derivatives of the Anti-HiBiT Monoclonal Antibody. These conjugates include XFD488 and XFD647, which are structurally identical to Alexa Fluor® dyes at the same wavelength. The fluorophore-conjugated antibodies are suitable for use in standard antibody-based detection protocols. This Technical Manual includes example protocols and representative data for FACS.
6/26