Wizard® Plus Minipreps DNA Purification System Technical Bulletin
Instructions for Use of Product(s)
A7100, A7141, A7500, A7510, A7211
Literature # TB117
The Wizard® Plus Minipreps DNA Purification System, which eliminates many of the problems associated with standard miniprep procedures, provides a simple and reliable method for rapidly isolating plasmid DNA. This system can be used to isolate any plasmid but works most efficiently when the plasmid is <20,000bp. When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Multiple minipreps may be easily processed at one time with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-sample capacity, Cat.# A7660) Laboratory Vacuum Manifold. DNA is eluted from the Wizard® Minicolumn in nuclease-free water. The purified plasmid can be used directly for automated fluorescent DNA sequencing.
Printed in USA. Revised 1/11.