HaloTag® Cloning Starter System
- Use Flexi® Cloning System to clone protein coding sequences into mammalian HaloTag® expression vectors
- Clone and transfer gene sequences into C- or N-terminus of HaloTag® Flexi® CMV Deletion Vectors
Catalog Number:
Size
Catalog Number: G6050
The HaloTag® Cloning Starter Kit is designed to utilize the Flexi® Cloning System to clone protein coding sequences into mammalian HaloTag® expression vectors. The system contains reagents required to clone and transfer gene sequences into the C- or N-terminus of a variety of HaloTag® Flexi® CMV Deletion Vectors.
This series of vectors containing deletions of human cytomegalovirus (CMV) immediate-early enhancer/promoter were created to provide different constitutive expression levels of HaloTag® 7 fusion proteins, providing greater control over the level of protein expression. This allows expression of enough fusion protein to image or capture but not so much to disturb normal function of the protein partner fused to the HaloTag® 7 protein.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
|
Wizard® SV Gel and PCR Clean-Up System |
A9280 | 1 × 10 preps | |
|
Flexi® System, Entry/Transfer |
C8641 | 1 × 1 each | View Product |
HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack |
G3780 | 1 × 9 × 2μg | View Product |
|
Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI) |
R1901 | 1 × 50μl | View Product |
SDS
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BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,420,367, 8,748,148, 9,593,316 and 9,873,866; European Pat. Nos. 2374875, 2492342 and 2502990; and Japanese Pat. Nos. 5680302 and 5840117.
For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.
Resources
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