Anti-NanoLuc® Monoclonal Antibody
Detects NanoLuc® Luciferase and NanoLuc® Fusion Proteins
- Affinity-purified mouse monoclonal antibody
- Use in Western blotting and immunofluorescence
Catalog Number:
Size
Catalog Number: N7000
Antibody to Detect NanoLuc® Luciferase
Anti-NanoLuc® Monoclonal Antibody can be used to detect NanoLuc® Luciferase or NanoLuc® fusion proteins by Western blotting and immunofluorescence. The Anti-NanoLuc® Antibody is a protein A/G affinity-purified mouse monoclonal antibody.
NanoLuc® luciferase is a small (19.1kDa), stable reporter enzyme that generates a bright luminescence signal. The small size and bright signal make NanoLuc® a versatile reporter that is used in many protein analysis applications. NanoLuc® fusion proteins are used for protein stability analysis and in NanoBRET® target engagement and protein interaction applications.
Western Blot Results
The figure on the right shows an example Western blot using HEK293 cells transfected with CMV-based expression constructs encoding NanoLuc® fusion proteins. The actual mobility approximated the expected mobility for each fusion protein. For Western blotting, we recommend a concentration of 1µg/ml as a starting point for protocol optimization. Background bands are evident in all lanes, including HEK293 cell lysate, which lacks expression of a NanoLuc® fusion protein, but the NanoLuc® fusion bands are more prominent.
Detection of NanoLuc® fusion proteins expressed in HEK293 cells.
Immunofluorescence Results
Shown below are example immunofluorescent images of HeLa cells transfected with a CMV-based construct expressing CDK2-NanoLuc® fusion protein. The CDK2-NanoLuc® fusion protein is stained red, and nuclei are marked blue via Hoechst staining. We recommend a concentration of 1-7.5µg/ml for immunofluorescence optimization. No NanoLuc® staining is observed in HeLa cells transfected with only carrier DNA (mock).
CDK2-NanoLuc®
(3µg/ml Anti-NanoLuc®)
NanoLuc®
Hoescht
Merge
Mock (5µg/ml Anti-NanoLuc®)
NanoLuc®
Hoescht
Merge
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
Anti-NanoLuc® Monoclonal Antibody |
N700A | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Related Products
Frequently Used With
NanoBRET® PPI Starter Systems
Live-cell protein:protein interaction assay. Includes vectors, controls and detection reagent for BRET-based PPI.
N1811, N1821
NanoBRET® Target Engagement Kinase Assays
Misurare direttamente l'affinità di legame chinasi-ligando, l'occupazione e il tempo di permanenza in cellule vive.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
Nano-Glo® Live Cell Assay
Measures NanoBiT® or NanoLuc® luminescence in living cells for up to 2 hours.
N2011, N2012, N2013
Nano-Glo® Luciferase Assay System
Add-mix-measure assay for NanoLuc® Luciferase with >2 hour half-life in most applications.
N1110, N1120, N1130, N1150
