FAO-Glo™ Fatty Acid Oxidation Assay
Rapid, Direct Measurement of Fatty Acid Oxidation (FAO)
- Direct detection of FAO activity, no surrogate markers
- Simple, plate-based protocol gives results in ~2 hours
- Reproducible results for confident decision making
- Amenable to high-throughput screening
Catalog Number:
Size
Catalog Number: CS3271A08
A Better Way to Measure FAO in Live Cells
Fatty acid oxidation, also known as beta-oxidation, is the metabolic process by which fatty acids are broken down in the mitochondria to generate energy. This process produces NADH and FADH2, which feed into the electron transport chain to drive ATP synthesis. FAO plays a critical role in cellular energy metabolism, especially during periods of fasting, exercise or metabolic stress, and is tightly linked to mitochondrial health and function. Understanding and measuring FAO is essential for studying metabolic diseases, obesity, diabetes and cancer.
Existing approaches to FAO measurement are either indirect, low throughput, or require specialized equipment. In contrast, the FAO-Glo™ Assay offers direct detection of FAO in a simple, plate-based format:
- No radioactivity
- No oxygen consumption proxies
- No expensive instrumentation.
It combines true functional analysis with scalability and speed for modern metabolism research.
How the FAO-Glo™ Assay Works
The FAO-Glo™ assay uses a proprietary FAO substrate that enters the mitochondria of live cells and undergoes fatty acid oxidation. During this process, FAO cycling triggers the release of luciferin, which is then quantified using a sensitive Luciferin Detection Reagent. Luminescence produced is proportionate to FAO occurring in the sample.
Reliable FAO Measurement from Low to High Cell Inputs
Cell number and time dependence of FAO. FAO-Glo™ was used to measure FAO in HEK293 cells for 1 hour with different amounts of cells in the presence or absence of 20mM etomoxir (A) or with 20K cells for different amounts of time (B).
Measuring FAO Inhibition and Activation
The FAO-Glo™ Assay successfully detected inhibition and activation of the pathway. HEK293 cells were treated with 20µM etomoxir, an inhibitor of FAO (left). HepG2 cells were treated with 10µM resveratrol, a known activator of FAO (right).
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
FAO Substrate |
CS3271A05 | 1 × 20μl |
FAO Luciferin Detection Reagent |
CS3271A06 | 1 × 10ml |
FAO Reconstitution Buffer |
CS3271A07 | 1 × 10ml |
d-Cysteine, 100X |
V251A | 1 × 100μl |
U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.
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