SpeI
- Capable of digesting DNA in 15 minutes or less
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
- Genome qualified to ensure optimal performance in genomic analysis applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6591
Catalog Number: R6595
Cut Site:
A▼CTAG T
T GATC▲A
Usage Note: Ends generated with SpeI can be directly ligated to ends generated by NheI or XbaI. None of these recognition sites are regenerated in the ligation product.
Incubation Conditions: Buffer B. 37°C.
Source: Sphaerotilus natans ATCC 13923.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | MULTI-CORE™ |
|---|---|---|---|---|
| 75–100% | 100% | 75–100% | 75–100% | 100% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 0 | 3 | 0 | 0 | 0 | 0 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer B 10X Buffer |
R002A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
SpeI |
R659A | 1 × 200u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer B 10X Buffer |
R002A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396D | 1 × 150μl | 10mg/ml | View Product |
|
SpeI |
R659D | 1 × 1,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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