HindIII
- Capable of digesting DNA in 15 minutes or less
- Active and capable of digestion directly in GoTaq® Green Master Mix
- Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications
Catalog Number:
Choose a concentration
Size
Catalog Number: R6041
Catalog Number: R6045
Cut Site:
A▼AGCT T
T TCGA▲A
Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/
Note: Star activity may be observed in the presence of Mn2+.
Incubation Conditions: Buffer E. 37°C.
Source: This enzyme is purified from an E. coli strain that carries the HindIII gene from Haemophilus influenzae Rd (ATCC 51907).
Storage Buffer: 10mM Tris-HCl (pH 7.3), 250mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.
Percent Activity in 4-CORE® Buffer:
| A | B | C | D | E | MULTI-CORE™ |
|---|---|---|---|---|---|
| 25–50% | 100% | 75–100% | 10–25% | 100% | 50–75% |
Frequency of Cutting:
| λ | Ad-2 | ΦX174 | pUC18 | M13mp18 | pBR322 |
|---|---|---|---|---|---|
| 7 | 12 | 0 | 1 | 1 | 1 |
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
HindIII |
R604A | 1 × 5,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Buffer E 10X Buffer |
R005A | 1 × 1ml | ||
|
Bovine Serum Albumin, Acetylated |
R396E | 1 × 750μl | 10mg/ml | View Product |
|
HindIII |
R604B | 1 × 15,000u | ||
|
MULTI-CORE™ 10X Buffer |
R999A | 1 × 0.25ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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