TransfectNow™ HEK293 Cells
Ready-to-Transfect HEK293 Cells
- No cell culture required prior to transfection
- Thaw-and-use cells
- Improved HEK293 cell transfection consistency
Catalog Number:
Size
Catalog Number: NC1001
Catalog Number: NC1002
Reliable, Thaw-and-Use Transfection-Ready Cells for Your Research
TransfectNow™ HEK293 Cells are ready-to-use for your transfection-based experiments—no cell culture required. Simply thaw, dilute and transfect.
Designed for use with the trusted FuGENE® HD Transfection Reagent, transient transfection with TransfectNow™ HEK293 Cells offers advantages over maintaining cells in continuous culture:
- Frees personnel from carrying out cell culture duties needed to achieve optimal transfections and maintain cell-line integrity.
- Saves time required to expand cells, allowing transfection-based experiments to be carried out sooner.
- Reduces consumable materials used for continuous cell culture, including growth media and plasticware.
- Robust quality control provides you with consistent and reliable transfections.
Save Time and Reduce Pre-Experiment Work
Start transfection experiments when you want without worrying about cell health or culture density. No need to spend multiple days for cell expansion prior to harvesting.
Go from Freezer to Results in One Day
After thawing and diluting TransfectNow™ HEK293 Cells, transfect your target-NanoLuc® fusion vector of interest in an assay plate. Cells demonstrate proven performance with NanoBRET® Target Engagement Assays and are ready the next day for testing your compounds using an addition-only protocol.
Achieve Successful Transfections with Less Effort
Quality-controlled manufacturing procedures ensure TransfectNow™ HEK293 Cells undergo consistent and high-efficiency transfections that are comparable to optimally cultured and harvested HEK293 cells.
TransfectNow™ HEK293 Cells are comparable to cultured HEK293 cells. Six Kinase-NanoLuc® fusion vector constructs were individually transfected in TransfectNow™ HEK293 Cells and freshly harvested HEK293 cells using FuGENE® HD Reagent. Each set of transfected cells was assayed using the NanoBRET® TE Intracellular Kinase Assay K-10 with or without a competing saturating dose of inhibitory compound (CC1 pan-kinase inhibitor) to compete with the BRET signal to determine the assay window. Total luminescence and BRET assay window were determined.
TransfectNow™ HEK293 Cells show day-to-day transfection efficiency. On two separate days, six Kinase-NanoLuc® fusion vector constructs were individually transfected in TransfectNow™ HEK293 Cells using FuGENE® HD Transfection Reagent. Each set of transfected cells was assayed using the NanoBRET® TE Intracellular Kinase Assay K-10 with or without a competing saturating dose of inhibitory compound (CC1 pan-kinase inhibitor) to compete with the BRET signal to determine the assay window. Total luminescence and BRET assay window were determined.
Successfully Transfect a Range of Fusion Vector Constructs
Comparable expression levels and BRET-based assay windows were obtained for 192 individual Kinase-NanoLuc® Fusion Vectors transfected using TransfectNow™ HEK293 Cells or freshly harvested HEK293 cells.
Cells transfected with Kinase-NanoLuc® fusion vectors were assayed using the NanoBRET® TE Intracellular Kinase Assay K-10 with or without a competing saturating dose of inhibitory compound (CC1 pan-kinase inhibitor) to determine BRET assay window. Panel A. Correlation plot shows that comparable NanoLuc® expression levels were obtained for all transfections with TransfectNow™ HEK293 Cells and freshly harvested HEK293 cells. Panel B. Comparable BRET-based assay windows were obtained for all transfections with TransfectNow™ HEK293 Cells and freshly harvested HEK293 cells.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
TransfectNow™ HEK293 Cells |
NC100A | 1 × 0.5ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
TransfectNow™ HEK293 Cells Limited Use Label License
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide recipient with a full refund.
This product and its transfected derivatives may not be further sold or transferred by recipient and may be used only by recipient, and then only for research use, which includes, but is not limited to, drug discovery and development. No commercial use is allowed. “Commercial use” means any and all uses of this product or its transfected derivatives by recipient for monetary or other consideration, including without limitation (1) product manufacturing, and (2) resale of this product or transfected derivatives for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of this product, except that recipient may transiently transfect these cells for use in generating research data. Notwithstanding the foregoing, recipient may use this product or transfected derivatives in provision of services to third parties in exchange for consideration (“Service Providers”); however, Service Providers may not transfer the product or transfected derivatives to third parties. Notwithstanding the transfer prohibitions of this limited use statement, end-user recipients, including, but not limited to non-profit recipients, and biotech and biopharma recipients, may transfer this product to third-party Service Providers, provided that Service Providers (1) receive a copy of this limited use statement and agree to comply with its terms; (2) use this product only on behalf of end-user recipient; (3) comply with the AdVec, Inc., license requirements described below; and (4) agree not to transfer this product or transfected derivatives to any other party.
Any use of this product by recipients who are for-profit entities (including without limitation biotechnology companies, biopharmaceutical companies and Service Providers) requires a license to HEK293 cell line from AdVec. Academic and other non-profit entities do not require any further license from AdVec. Unlicensed for-profit entities may contact AdVec, Dr. Frank Graham, CEO, at graham@mcmaster.ca.
No other use of this product is authorized without the prior express written consent of Promega. With respect to any uses outside this limited use statement, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
HEK293 cells were obtained under license from AdVec Inc.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
TransfectNow™ HEK293 Cells |
NC100B | 2 × 1ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
TransfectNow™ HEK293 Cells Limited Use Label License
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If recipient is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide recipient with a full refund.
This product and its transfected derivatives may not be further sold or transferred by recipient and may be used only by recipient, and then only for research use, which includes, but is not limited to, drug discovery and development. No commercial use is allowed. “Commercial use” means any and all uses of this product or its transfected derivatives by recipient for monetary or other consideration, including without limitation (1) product manufacturing, and (2) resale of this product or transfected derivatives for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of this product, except that recipient may transiently transfect these cells for use in generating research data. Notwithstanding the foregoing, recipient may use this product or transfected derivatives in provision of services to third parties in exchange for consideration (“Service Providers”); however, Service Providers may not transfer the product or transfected derivatives to third parties. Notwithstanding the transfer prohibitions of this limited use statement, end-user recipients, including, but not limited to non-profit recipients, and biotech and biopharma recipients, may transfer this product to third-party Service Providers, provided that Service Providers (1) receive a copy of this limited use statement and agree to comply with its terms; (2) use this product only on behalf of end-user recipient; (3) comply with the AdVec, Inc., license requirements described below; and (4) agree not to transfer this product or transfected derivatives to any other party.
Any use of this product by recipients who are for-profit entities (including without limitation biotechnology companies, biopharmaceutical companies and Service Providers) requires a license to HEK293 cell line from AdVec. Academic and other non-profit entities do not require any further license from AdVec. Unlicensed for-profit entities may contact AdVec, Dr. Frank Graham, CEO, at graham@mcmaster.ca.
No other use of this product is authorized without the prior express written consent of Promega. With respect to any uses outside this limited use statement, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING AS TO MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
HEK293 cells were obtained under license from AdVec Inc.
Resources
Other
- Product Application: TransfectNow™ HEK293 Cells with NanoBRET™ Target Engagement Assays in a Nonbinding Surface Format
- Product Application: TransfectNow™ HEK293 Cells with NanoBRET™ Protein:Protein Interaction System
- Product Application: TransfectNow™ HEK293 Cells with NanoBiT® Protein:Protein Interaction System
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Frequently Used With
FuGENE® HD Transfection Reagent
Enhanced performance in a range of cell types, including difficult-to-transfect cell lines.
E2311, E2312
NanoBRET® Target Engagement Kinase Assays
Misurare direttamente l'affinità di legame chinasi-ligando, l'occupazione e il tempo di permanenza in cellule vive.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
NanoBRET® Target Engagement CDK Selectivity Systems
Profilo della selettività degli inibitori per le chinasi ciclina-dipendenti in cellule vive.
NP5000, NP5050, NP5100, NP5150
NanoBRET® Target Engagement K192 Kinase Selectivity System
Misurare quantitativamente l'occupazione intracellulare dei composti contro un gruppo di 192 chinasi in un singolo esperimento.
NP4050, NP4060, NP4100, NP4101
