NanoBRET® 590 Dyes
Develop Novel NanoBRET® Tracers to Enable Target Engagement Assays for New Targets
- Leverage the same NanoBRET®-branded energy acceptors used to create most Promega NanoBRET® tracers
- Couple NanoBRET® 590 SE Dyes to base compounds containing a reactive amine group
- Create three unique tracer structures from a common starting material using the three linker variants of the NanoBRET® 590 SE Dye
- Synthesize tracers with an amine orthogonal coupling chemistry using Azide-Alkyne Click chemistry and the NanoBRET® 590 Azide Dye
Catalog Number:
Choose a dye
Size
Catalog Number: N5000
Catalog Number: N5001
Catalog Number: N5100
Catalog Number: N5200
Catalog Number: N5201
Catalog Number: N5300
Catalog Number: N5301
Creazione di traccianti NanoBRET™ TE per il target di interesse
Coloranti NanoBRET™ 590 utilizzati nel flusso di lavoro per la scoperta di farmaci in fase iniziale
Le librerie codificate a DNA (DEL) forniscono una diversità chimica senza pari e punti di partenza per nuove modalità di farmaci. Un articolo pubblicato su Cell Chemical Biology presenta un flusso di lavoro che sfrutta gli attributi bifunzionali dei ligandi DEL come piattaforma per generare sonde BRET per studi di aggancio del bersaglio in cellule vive. La combinazione della tecnologia DEL e BRET accelera gli studi hit-to-lead in ambiente live-cell.
Per ulteriori informazioni, vedere: Teske, K.A. et al. (2023) I DEL consentono lo sviluppo di sonde BRET per studi di aggancio del bersaglio nelle cellule. Cell Chem. Biol. 30, 1-12.
Il NanoBRET™ Target Engagement (TE) Assay misura il legame di composti a proteine target selezionate all'interno di cellule intatte. Questo saggio si basa sul sistema NanoBRET™, una tecnica di trasferimento energetico progettata per misurare la prossimità molecolare nelle cellule vive. Il NanoBRET™ Target Engagement Assay analizza l'affinità apparente dei composti in esame attraverso lo spostamento competitivo di un tracciante NanoBRET™ legato reversibilmente a una proteina di fusione NanoLuc® in una cellula.
Abbiamo già sviluppato una serie di saggi TE per un gran numero di target proteici appartenenti a diverse classi di proteine. Se non esiste un saggio NanoBRET™ TE esistente per il target di interesse, è possibile utilizzare i coloranti NanoBRET™ 590 per sviluppare nuovi traccianti che consentano di eseguire saggi NanoBRET™ TE per nuovi target.
Come funziona il test NanoBRET™ TE
Illustrazione del test NanoBRET™ TE. Pannello A. Il legame del composto viene misurato in un formato competitivo utilizzando un tracciante NanoBRET™ fluorescente permeabile alle cellule. Il legame del composto in esame determina una perdita di segnale NanoBRET™ tra la proteina bersaglio e il tracciante all'interno di cellule intatte. Pannello B. L'affinità del tracciante NanoBRET™ è determinata per ciascuna proteina bersaglio. Per l'analisi dell'aggancio del bersaglio da parte di un composto di prova, le cellule vengono trattate con una concentrazione fissa di tracciante NanoBRET™ che si avvicina al valore EC50 della curva dose-risposta del tracciante NanoBRET™. Pannello C. Per determinare l'affinità del composto di prova, le cellule vengono titolate con concentrazioni variabili del composto di prova in presenza di una concentrazione fissa (EC50-EC80) del tracciante.
I coloranti contenenti linker accelerano lo sviluppo di traccianti
I coloranti NanoBRET™ 590 possono essere utilizzati per sviluppare nuovi traccianti permeabili alle cellule e sono gli stessi utilizzati per creare i traccianti NanoBRET™ disponibili presso Promega. I coloranti NanoBRET™ 590 hanno dimostrato una solida permeabilità cellulare e una sovrapposizione spettrale ottimale con la luciferasi NanoLuc® per il trasferimento di energia. Sono disponibili tre varianti di linker dei coloranti NanoBRET™ 590 SE(pannelli A-C), che consentono di preparare un pannello di candidati traccianti da un intermedio comune e forniscono la diversità strutturale necessaria per garantire la massima probabilità di successo e, se necessario, per guidare l'ulteriore ottimizzazione dello sviluppo del tracciante. È inoltre disponibile il colorante azidico NanoBRET™ 590(pannello D), che consente la sintesi del tracciante mediante chimica azide-alchinica a click e fornisce una chimica di accoppiamento ortogonale con l'ammina.
Struttura dei coloranti NanoBRET™ (fluoroforo in rosso). Pannello A. Colorante NanoBRET™ 590 SE (MW: 426,2g/mol). Pannello B. Colorante NanoBRET™ 590 C4 SE (MW: 511,3g/mol). Pannello C. Colorante NanoBRET™ 590 PEG(O4) SE (MW: 673,5g/mol). Pannello D. Colorante NanoBRET™ 590 C3 Azide (MW: 411,2g/mol).
Trasformate i vostri traccianti in saggi
Se non esiste un saggio NanoBRET™ TE esistente per il target di interesse, i coloranti NanoBRET™ 590 possono essere utilizzati per sviluppare nuovi traccianti che consentano di eseguire saggi NanoBRET™ TE per nuovi target.
Flusso di lavoro per lo sviluppo del saggio NanoBRET™ TE
Flusso di lavoro per lo sviluppo di un saggio NanoBRET™ TE. Le tre fasi di realizzazione di un NanoBRET™ TE Assay sono: (1) sviluppare un tracciante fluorescente che leghi il target di interesse; (2) costruire un vettore che esprima una fusione della proteina target con la luciferasi NanoLuc® nelle cellule; (3) ottimizzare il saggio cellulare NanoBRET™ TE utilizzando i reagenti del saggio NanoBRET™ TE. Per completare un flusso di lavoro completo sono necessari altri reagenti; per saperne di più, consultare i nostri prodotti di uso frequente (sotto).
Nella letteratura
Progettazione, sintesi e valutazione di degradatori della proteina 5 contenente WD-Repeat (WDR5)
Questo studio presenta la progettazione, la sintesi e la valutazione di due diverse serie di degradatori di WDR5 basate su due scaffold che legano il sito WIN e dimostra che la natura e la lunghezza del linker influenzano fortemente l'efficacia della degradazione.
La modulazione delle chinasi della tubulina tau (TTBK1 e TTBK2) influisce sulla ciliogenesi
Data l'importanza biologica di queste chinasi, gli autori hanno progettato una libreria mirata dalla quale hanno identificato diversi strumenti chimici che coinvolgono TTBK1 e TTBK2 nelle cellule e inibiscono la loro segnalazione a valle.
Misurazioni quantitative e in tempo reale del coinvolgimento del bersaglio intracellulare mediante trasferimento di energia
Il trasferimento di energia di risonanza della bioluminescenza (BRET) offre una soluzione per l'aggancio del bersaglio intracellulare quando sono necessarie metriche quantitative o analisi cinetiche.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 SE |
N500A | 1 × 5mg |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 SE |
N500B | 1 × 25mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 Azide |
N510A | 1 × 5mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 PEG(O4)-SE |
N520A | 1 × 5mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 SE |
N500A | 1 × 5mg |
NanoBRET® 590 PEG(O4)-SE |
N520A | 1 × 5mg |
NanoBRET® 590 C4 SE |
N530A | 1 × 5mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 C4 SE |
N530A | 1 × 5mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
NanoBRET® 590 C4 SE |
N530B | 1 × 25mg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® Dyes Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only, which shall include, without limitations, researcher’s (a) use of the product in its research and development of commercial products (provided that no such product requires the practice of Promega technology) (“Researcher Products”) or (b) use of any data generated using the product for the regulatory approval of such Researcher Products.
Researcher may not modify the product except to link the product to Promega or other non-Promega moieties (“Derivatives”).
For uses of this product and/or Derivatives for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(1a) use NanoLuc®-branded or NanoBiT®-branded luciferase, subject to any relevant label licenses, for all energy transfer determinations conducted with this product and/or Derivatives; and
(1b) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate, Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor) manufactured by Promega for all determinations of luminescent activity; or
(1c) contact Promega to obtain a license for use of the product or Derivatives with luciferases or LARs not manufactured by Promega.
Except as outlined in (2a) or (2b) below, researcher has no right to i) sell or transfer the product and/or Derivatives to another entity; (ii) sell data or information generated using the product and/or Derivatives to another entity; or iii) sell any product manufactured using the product and/or Derivatives to another entity.
(2a) If researcher is a contract research organization (“CRO”), such CRO may use the product solely on behalf of a single CRO customer to generate Derivatives and transfer or sell such Derivatives or data or information generated using the product and/or Derivatives to the single CRO customer, provided that CRO provides its single customer with a copy of this label license with the transfer of any Derivatives and the CRO customer agrees to comply with this label license for any use of the Derivatives; or
(2b) If researcher is not a CRO, researcher may transfer this product and/or Derivatives to a CRO in order to have CRO generate Derivatives solely on behalf of researcher or have CRO use Derivatives to provide researcher information, data or new Derivatives, and CRO may transfer the information, data or Derivatives to researcher, provided that researcher provides CRO with a copy of this label license and CRO agrees to comply with the terms of this label license.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic, or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Resources
Articles
- Finding the Nexus: A New Technique for Studying Kinase Target Engagement in Live Cells
- Advancing Biomedical Research with Quality Chemical Probes
- Blog Article: The Surprising Landscape of CDK Inhibitor Selectivity in Live Cells
- Development of a HiBiT Peptide-Based NanoBRET Ligand Binding Assay for Galanin Receptor 1 in Live Cells
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