Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor
For use with NanoBRET® Target Engagement Intracellular Assays over Multiple Hours
- Supplies the Nano-Glo® Vivazine® Substrate and Extracellular NanoLuc® Inhibitor
- Optimal for experiments >2 hours
- Ensures measurements originate from inside intact cells
Catalog Number:
Size
Catalog Number: N2200
Catalog Number: N2201
Intracellulare TE Nano-Glo® Vivazine™/Inibitore
L'Intracellular TE Nano-Glo® Vivazine™/Inhibitor è progettato per l'uso con i test NanoBRET™ Target Engagement (TE), che misurano in tempo reale il legame di composti a proteine target selezionate in cellule intatte. Il saggio NanoBRET™ TE utilizza quattro componenti chiave: una proteina bersaglio cellulare espressa fusa con la luciferasi NanoLuc® luciferasi; un tracciante fluorescente permeabile alla cellula che si lega specificamente alla proteina target; un substrato per la NanoLuc® luciferasi; e un inibitore cellula-impermeabile per la NanoLuc® luciferasi.
L'Intracellular TE Nano-Glo® Vivazine™/Inhibitor è stato progettato per misurazioni NanoBRET™ TE in cellule di durata superiore alle 2 ore. Contiene il substrato Nano-Glo® Vivazine™ e l'inibitore extracellulare NanoLuc®. Il substrato Nano-Glo® Vivazine™ è un substrato NanoLuc a durata prolungata, progettato per fornire una luce brillante.® progettato per fornire un segnale luminescente luminoso per misurazioni end-point o esperimenti cinetici in tempo reale. L'inibitore extracellulare NanoLuc® Extracellular NanoLuc è una molecola permeabile alle cellule, progettata per spegnere il segnale NanoLuc® derivato dall'ambiente extracellulare o da cellule compromesse.
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
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