Intracellular TE Nano-Glo® Substrate/Inhibitor
For use with NanoBRET® Target Engagement Intracellular Assays
- Supplies the NanoBRET® Nano-Glo® Substrate and Extracellular NanoLuc® Inhibitor
- Optimal for experiments ≤2 hours
- Ensures measurements originate from inside intact cells
Catalog Number:
Size
Catalog Number: N2162
Catalog Number: N2160
Catalog Number: N2161
Il substrato/inibitore Intracellular TE Nano-Glo® è utilizzato per i saggi di coinvolgimento del bersaglio (TE) intracellulari NanoBRET™. Il substrato/inibitore Intracellulare TE Nano-Glo® Substrato/Inibitore contiene due componenti: il substrato NanoBRET™ Nano-Glo® e l'inibitore NanoLuc extracellulare.® inibitore. Il substrato NanoBRET™ Nano-Glo® è il substrato utilizzato dalla NanoLuc® Luciferasi per generare il segnale donatore in diversi dosaggi NanoBRET™. L'inibitore extracellulare NanoLuc® Extracellular NanoLuc è usato per sopprimere qualsiasi segnale NanoLuc® all'esterno delle cellule. L'inibitore garantisce quindi che le misurazioni NanoBRET™ provengano dall'interno di cellule intatte.
Il substrato/inibitore intracellulare TE Nano-Glo® è disponibile separatamente per integrare i seguenti sistemi:
- NanoBRET™ Target Engagement HDAC Assays
- NanoBRET™ Target Engagement BET BRD Assays
- NanoBRET™ Target Engagement Kinase Assays
Inoltre, il substrato/inibitore Intracellular TE Nano-Glo® è utilizzato con i traccianti di aggancio del bersaglio intracellulare NanoBRET™ realizzati con i coloranti NanoBRET™ (che possono essere richiesti ai nostri Servizi di analisi personalizzata). Per ulteriori informazioni, si prega di chiedere informazioni.
Misurare l'affinità chinasi-composto in cellule vive
Per saperne di più su NanoBRET™ Kinase Target EngagementSpecifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157A | 1 × 50μl | View Product |
Extracellular NanoLuc® Inhibitor |
N235A | 1 × 17μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157C | 1 × 330μl | View Product |
Extracellular NanoLuc® Inhibitor |
N235B | 1 × 110μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
NanoBRET® Nano-Glo® Substrate |
N157D | 1 × 3.3ml | View Product |
Extracellular NanoLuc® Inhibitor |
N235C | 1 × 1.1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE LAR Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than genetically encoded autofluorescent protein, researcher must:
(i) use NanoBRET®-branded energy acceptors (e.g., NanoBRET® tracers, NanoBRET® dyes, BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity; or
(ii) contact Promega to obtain a license for use of the product for energy transfer assays using energy acceptors not manufactured by Promega.
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
Resources
Related Products
Frequently Used With
NanoBRET® Target Engagement Kinase Assays
Misurare direttamente l'affinità di legame chinasi-ligando, l'occupazione e il tempo di permanenza in cellule vive.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
NanoBRET® Target Engagement BET BRD Assays
Directly measure BET bromodomain-test compound affinity and residence time in live cells.
N2131, N2180, N2130, N2140
NanoBRET® Target Engagement HDAC Assays
Directly measure HDAC-test compound affinity and residence time in live cells.
N2080, N2081, N2170, N2090
