DDR1-NanoLuc® Fusion Vector
Kinase Fusion Vector for NanoBRET® Target Engagement
- Transfection-ready DNA to express full-length DDR1 kinase fused to NanoLuc® luciferase
- Measures kinase target engagement in live cells
- Supplied as a control vector with NanoBRET® TE Intracellular Kinase Assay
Catalog Number:
Size
Catalog Number: N2451
Esprimere fusioni NanoLuc®-Kinase a lunghezza piena in cellule di mammifero
Il vettore di fusione DDR1-NanoLuc® contiene la regione codificante di NanoLuc® luciferasi fusa con il C-terminus della chinasi DDR1 umana. È stato progettato per l'uso con il NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay, K-4, dove il plasmide può essere trasfettato in varie linee cellulari per l'analisi del target BTK. Il vettore di fusione DDR1-NanoLuc® è disponibile per l'acquisto come vettore a sé stante, ma è anche fornito come DNA di controllo nel kit NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay, K-4. I vettori di fusione della chinasi NanoLuc® luciferasi sono forniti pronti per la trasfezione. Il promotore CMV guida l'espressione della proteina di fusione NanoLuc® chinasi a lunghezza completa.
Dettagli del vettore:
Informazioni sui nomi dei vettori di fusione delle chinasi
Il posizionamento di NanoLuc® a 5´ o 3´ del gene della chinasi è ottimizzato per l'uso con i saggi NanoBRET™ TE e si riflette nel nome del vettore. Ad esempio, NanoLuc®-kinase implica che NanoLuc® si trova a 5´ del gene della chinasi (all'N-terminus della chinasi). Analogamente, kinase-NanoLuc® implica che NanoLuc® si trova a 3' dal gene della chinasi (al C-terminus della chinasi).
Saggi di chinasi intracellulare NanoBRET™ Target Engagement (TE)
I saggi di chinasi intracellulare NanoBRET™ TE possono analizzare l'affinità dei composti in esame mediante lo spostamento competitivo di un tracciante fluorescente NanoBRET™ legato reversibilmente a una proteina di fusione NanoLuc®- chinasi nelle cellule. L'introduzione di composti di prova concorrenti determina una diminuzione dose-dipendente del segnale NanoBRET™ per stimare l'affinità intracellulare contro la proteina bersaglio. Il tracciante NanoBRET™ utilizzato è determinato dalla scelta del vettore di fusione della chinasi NanoLuc®. La tabella di selezione del vettore chinasi mostra quale saggio NanoBRET™ Tracer è corretto per ciascun vettore di fusione chinasi.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
DDR1-NanoLuc® Fusion Vector |
N245A | 1 × 20μg | 1μg/μl | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researchers may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researcher may (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence; and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use of this product or derivatives is authorized without the prior express written consent of Promega.
In addition, researchers must:
(1a) use Nano-Glo®-branded luminescent assay reagents (LARs) for all determinations of luminescence activity of this product and its derivatives; or
(1b) contact Promega to obtain a license for use of the product and its derivatives with LARs not manufactured by Promega.
For uses of Nano-Glo®-branded LARs intended for energy transfer (such as bioluminescence resonance energy transfer) to acceptors other than a genetically encoded autofluorescent protein, researchers must:
(2a) use NanoBRET®-branded energy acceptors (e.g., BRET-optimized HaloTag® ligands) for all determinations of energy transfer activity by this product and its derivatives; or
(2b) contact Promega to obtain a license for use of the product and its derivatives for energy transfer assays to energy acceptors not manufactured by Promega.
Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. Nos. 8,557,970, 8,669,103, 9,777,311, 9,840,730, 9,951,373, 10,233,485, 10,633,690, 10,774,364, 10,844,422, 11,365,436, 11,661,623, 11,667,950; European Pat. Nos. 2456864, 2635595, 2990478, 3181687, 3409764; Japanese Pat. Nos. 6038649, 6155424, 6227615, 6374420, 6539689; and other patents and patents pending.
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