Cell-Permeable Kinase NanoBRET® TE Tracers
Measure Cellular Affinity of Compounds for Kinase Targets in Live Cells
- Part of NanoBRET® Target Enhancement (TE) Intracellular Kinase Assays
- NanoBRET® TE Tracer products increase flexibility and modularity of NanoBRET® TE Kinase Assays
- Use with Intracellular TE Nano-Glo® substrate/inhibitor and NanoLuc®-kinase fusion vectors
Catalog Number:
Choose a tracer
Size
Catalog Number: N2602
Catalog Number: N2482
Catalog Number: N2492
Catalog Number: N2622
Catalog Number: N2632
Catalog Number: N2642
Catalog Number: N2652
Catalog Number: NF1011
Misurare l'affinità chinasi-ligando in cellule vive
Un tracciante NanoBRET™ è composto da un ligando o inibitore biodisponibile di una piccola molecola di una proteina bersaglio, coniugato covalentemente con un colorante fluorescente. Diversi traccianti NanoBRET™ sono stati sviluppati come parte del sistema NanoBRET™ TE Intracellular Kinase Assay, che utilizza un metodo basato sul trasferimento di energia di risonanza bioluminescente (BRET) per misurare il legame dei composti a determinate chinasi target all'interno di cellule intatte.
In particolare, i saggi NanoBRET™ TE Kinase consentono di analizzare l'affinità apparente cellulare dei composti in esame mediante lo spostamento competitivo di un tracciante NanoBRET™ permeabile alle cellule e legato reversibilmente a una proteina di fusione chinasi-NanoLuc® luciferasi nelle cellule. La proteina di fusione chinasi-NanoLuc® serve come donatore di energia, mentre il tracciante fluorescente NanoBRET™ TE serve come accettore di energia nel saggio BRET.
I traccianti NanoBRET™ TE possono essere acquistati singolarmente o come parte di un saggio di chinasi intracellulare NanoBRET™ TE. Ogni NanoBRET™ TE Kinase Assay fornisce i reagenti necessari per il rilevamento NanoBRET™, tra cui un tracciante NanoBRET™, un substrato NanoBRET™ Nano-Glo® Substrato, NanoLuc extracellulare® inibitore e tampone di diluizione del tracciante. I traccianti NanoBRET™ TE offrono agli utenti una maggiore flessibilità per studiare nuove chinasi o testare traccianti alternativi.
I vettori di fusione chinasi-NanoLuc® sono disponibili separatamente. Il tracciante NanoBRET™ e la concentrazione raccomandata per una proteina di fusione kinasi-NanoLuc selezionata sono riportati in un documento.® per una proteina di fusione chinasi-NanoLuc selezionata sono riportate in una nota applicativa here.
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Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-3 |
N260B | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-5 |
N248B | 1 × 550μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-4 |
N249B | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-8 |
N262B | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-9 |
N263B | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-10 |
N264B | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-11 |
N265B | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
What's in the box
| Item | Part # | Choose a size | Concentration |
|---|---|---|---|
NanoBRET® Tracer K-12 |
NF101A | 1 × 300μl | 400μM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
NanoBRET® TE Tracers Limited Use Label License
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no commercial use is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product, whether or not such product is resold for use in research. Researcher shall have no right to modify or otherwise create variations of the product. No other use or transfer of this product is authorized without the prior express written consent of Promega.
For uses of this product for energy transfer (such as bioluminescence resonance energy transfer), researcher must:
(i) use NanoLuc®-branded luciferase or derivatives for all energy transfer determinations conducted with this product; and
(ii) use NanoBRET®-branded luminescent assay reagents (LARs; e.g., NanoBRET® Nano-Glo® Substrate), Intracellular TE Nano-Glo® Substrate/Inhibitor or Intracellular TE Nano-Glo® Vivazine®/Inhibitor for all determinations of luminescent activity; or
(iii) contact Promega to obtain a license for use of the product with LARs not listed above or manufactured by Promega.
In addition, researcher must:
Contact Promega to obtain a license for use of the product in assays not involving bioluminescence resonance energy transfer (BRET).
With respect to any uses outside this label license, including any diagnostic, therapeutic, prophylactic or commercial uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, WITH REGARD TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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Related Products
Frequently Used With
Intracellular TE Nano-Glo® Substrate/Inhibitor
Il substrato NanoLuc® e l'inibitore extracellulare raccomandati per i saggi intracellulari NanoBRET™ Target Engagement.
N2162, N2160, N2161
NanoBRET® Target Engagement Kinase Assays
Misurare direttamente l'affinità di legame chinasi-ligando, l'occupazione e il tempo di permanenza in cellule vive.
N2520, N2521, N2540, N2501, N2500, N2530, N2600, N2601, N2620, N2621, N2630, N2631, N2640, N2641, N2650, N2651, NF1001, N2810, N2820, N2830, N2840, N2850, NF1200
Intracellular TE Nano-Glo® Vivazine® Substrate/Extracellular NanoLuc® Inhibitor
Un substrato NanoLuc® alternativo e un inibitore extracellulare da utilizzare nei saggi intracellulari NanoBRET™ Target Engagement che prevedono un monitoraggio cinetico esteso.
N2200, N2201
