CCNA1 Expression Vector

NV2591_CCNA1 Expression Vector- 20ug
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Regulatory Vector for NanoBRET® Target Engagement

  • Transfection-ready DNA to express full-length CCNA1 regulatory protein
  • Use with select kinase-NanoLuc® fusion vectors and NanoBRET® TE Intracellular Kinase Assay

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Catalog number selected: NV2591

$ 585.00
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CCNA1 Expression Vector
3 × 20μg
$ 585.00
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Express Regulatory Proteins in Mammalian Cells

The CCNA1 Expression Vector contains the coding region of the human CCNA1 regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The Expression Vector and kinase-NanoLuc® Fusion Vector are cotransfected into cells prior to analysis using the appropriate NanoBRET® Target Engagement Intracellular Kinase Assay. These regulatory protein Expression Vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.

For details on kinase-NanoLuc® Fusion Vectors that require co-expression with a regulatory protein Expression Vector, see the NanoBRET® Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.

Vector Details:

  • Gene name: CCNA1
  • Sequence
  • Recommended Assay: NanoBRET® TE Intracellular Kinase Assay, Adherent Format

About Kinase Fusion Vector Names

The placement of NanoLuc® either 5´ or 3´ of the kinase gene is optimized for use with NanoBRET® TE Assays and reflected in the vector name. For example, NanoLuc®-kinase implies that NanoLuc® is 5´ to the kinase gene (at the N-terminus of the kinase). Similarly, kinase-NanoLuc® implies NanoLuc® is 3´to the kinase gene (at the C-terminus of the kinase).

NanoBRET® Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET® TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET® Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET® signal for estimating intracellular affinity against the target protein. The NanoBRET® Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET® Tracer assay is correct for each kinase fusion vector.

Specifications

What's in the box?

Item Part # Size Concentration

CCNA1 Expression Vector

NV259A 3 × 20μg 1μg/μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

Licensed from Kazusa Genome Technologies.

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