Transfection Carrier DNA
Plasmid DNA Used to Dilute Expression or Reporter Vectors for Transfection
- Maintains the total amount of DNA used in transfection while reducing the amount of expression or reporter vector
- Lowered expression of the protein of interest can overcome potential overexpression artifacts
- Ratio of carrier DNA to reporter DNA may need to be optimized, depending on the application
- Supplied at a concentration of 1µg/μl
Catalog Number:
Size
Catalog Number: E4881
Catalog Number: E4882
Il DNA vettore di trasfezione è un DNA plasmidico utilizzato per ridurre la quantità di un vettore di espressione o di un vettore reporter nella trasfezione di cellule di mammifero senza ridurre la quantità complessiva di DNA. La diluizione del DNA reporter nel DNA vettore di trasfezione determina una minore espressione della proteina di interesse, superando gli artefatti dovuti alla sovraespressione. Ciò è particolarmente importante quando si utilizzano promotori forti e costitutivi (ad esempio, CMV). A seconda dell'applicazione, il rapporto tra DNA reporter e DNA vettore di trasfezione può richiedere un'ottimizzazione.
Questo prodotto è ricavato dal vettore pGEM®-3Zf(-), ma è stato purificato con un metodo che determina un basso carryover di endotossine, rendendolo più adatto all'uso nella trasfezione di cellule di mammifero.
Protocols
No protocols available
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
Transfection Carrier DNA |
E488A | 5 × 20μg | 1μg/μl | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
Transfection Carrier DNA |
E488B | 2 × 100μg | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
No related resources available
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