pGEM®-4Z Vector
Standard Cloning Vector That can also Efficiently Synthesize RNA In vitro
- Blue/white screening to easily identify recombinant clones
- Versatile vector used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
- Multiple cloning site provides a convenient selection of restriction sites for cloning
Catalog Number:
Size
Catalog Number: P2161
The pGEM®-4Z Vector is a standard cloning vector that can also efficiently synthesize RNA in vitro. The vector carries the lacZ α-peptide and the multiple cloning region arrangement from pUC18 for selecting recombinants by blue/white screening. In addition, the vector contains both the SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
The pGEM®-3Z and pGEM®-4Z Vectors are essentially identical except for the orientation of the SP6 and T7 promoters.
Reference
- Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
pGEM®-4Z Vector GenBank® Accession Number X65305 and vector sequence text file.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
pGEM®-4Z Vector |
P216A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for storage recommendations.
Resources
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Frequently Used With
JM109 Competent Cells
E. coli strain ready for transformation; offers blue/white selection.
L2005, L1001, L2001
HB101 Competent Cells
E. coli strain ready for transformation. No blue/white selection.
L2015, L2011
Riboprobe® Systems
Generate high-specific-activity RNA probes or transcripts; choice of SP6, T7 or T3 systems.
P1420, P1430, P1440
RiboMAXâ„¢ Large-Scale RNA Production Systems
Compatible with modified or unmodified nucleotides. Produces 2–5mg/ml RNA.
P1280, P1300
