HaloTag® Mammalian Protein Detection and Purification Systems Technical Manual

Literature # TM348

The HaloTag® Mammalian Protein Purification System is based on the HaloTag® technology. HaloTag® is a protein fusion tag engineered to form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes the HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of HaloTag® fusion proteins facilitates rapid optimization of expression and purification conditions.