QC Tests
Activity, DNase, RNase, endonuclease, cDNA synthesis.
Source
Recombinant E. coli strain.
Storage Buffer
20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 50% (v/v) glycerol, 0.2mg/ml BSA.
Storage Conditions
Store at –20°C.
Unit Definition
One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).
For product intended use please see Patents & Disclaimers tab.
Use Restrictions
M4281, M4285 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.