Gradual nonenzymatic deamidation of asparagine or rearrangement of aspartic acid residues during storage or handling can result in isoaspartic acid residues 70–85% of the time.
ISOQUANT® Isoaspartate Detection Kit
This kit uses a sensitive, nonradioactive HPLC method for detecting and quantifying deamidated peptides.
Mass Spectrometry Analysis
Proteases for optimal digestion in mass spectrometry or liquid chromatography applications including trypsin, chymotrypsin, rLys-C, Lys-C, Asp-N and Glu-C.
Trypsin Gold, Mass Spectrometry Grade
Manufactured for maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active, stable molecule that is extremely resistant to autolytic digestion. Specificity is further improved by TPCK treatment. Each lot is qualified for in-gel digestion and mass spectrometric analysis.
Custom Mass Spectrometry Services
Promega Corporation and MS Bioworks have formed a collaboration that gives researchers access to a complete workflow solution, by providing unique proteomic reagents coupled with mass spectrometry services.
An article showing how Arg-C, elastase, thermolysin and pepsin can improve digestion of proteins that lack trypsin cleavage sites, or resist or block trypsin digestion.
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