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15N Labeling of Proteins Overexpressed in the Escherichia coli Strain KRX

Kate Qin Zhao, Jim Hartnett and Michael R. Slater
Promega Corporation
Publication Date: 2009

Abstract

We describe a protocol for efficient 15N-labeled protein production in the E. coli strain, KRX. This procedure can help streamline the process of target cloning, protein expression, screening and preliminary protein folding/aggregation assessment by NMR spectroscopy and other methods that require 15N protein labeling.

Promega Notes 97, 28–29.

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