PowerPlex® 35GY System for Use on the Spectrum Compact CE System Technical Manual
Instructions for Use of Product(s)
DC3520
Literature # TMD076
The PowerPlex® 35GY System is a 35-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. This eight-color system allows co-amplification and fluorescent detection of the 20 autosomal loci in the expanded CODIS core loci (CSF1PO, FGA, TH01, TPOX, vWA, D1S1656, D2S1338, D2S441, D3S1358, D5S818, D7S820, D8S1179, D10S1248, D12S391, D13S317, D16S539, D18S51, D19S433, D21S11 and D22S1045) as well as Amelogenin and DYS391 for gender determination. The Penta D, Penta E, and SE33 loci are also included to increase discrimination and allow searching of databases that include profiles with these loci. Finally, ten additional Y-STR loci, DYS385ab, DYS390, DYS438, DYS448, DYS456, DYS458, DYS481, DYS635 and DYS643 are included in the multiplex. This extended panel of STR markers is intended to satisfy both CODIS and ESS recommendations. This manual contains a protocol to separate amplified products and detect separated material on the Spectrum Compact CE System.
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