IL-23 Bioassay Technical Manual
Instructions for Use of Product(s)
JA2511, JA2515, JA3511, JA3515
Literature # TM609
The IL-23 Bioassay is a bioluminescent cell-based assay designed to measure IL-23 stimulation or inhibition. The IL-23 Bioassay Cells are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-23 Bioassay consists of human cells engineered to express the IL-23 receptor and a luciferase reporter driven by a response element (RE). When IL-23 binds to IL-23R, intracellular signals are transduced, resulting in luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 6/26 revision of this document:
1. Removed Section 8, Related Products.
2. Made minor text and formatting edits.
Revised 6/26.