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Notice: Promega will be performing essential system updates January 21–25.

Order processing and shipping will pause starting January 21 at 4:00 PM CST and resume on January 26.
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Please contact Customer Service with any questions.

Reverse Transcription System Technical Bulletin

Instructions for Use of Product(s)
A3500

Literature # TB099

AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ isolated RNA. The Reverse Transcription System provides lot-tested reagents to efficiently reverse transcribe poly(A)+ mRNA or total RNA in 15 minutes. A polyadenylated 1.2kb transcript is provided as a control template for the cDNA synthesis reaction. cDNA synthesized with the Reverse Transcription System can be used directly in PCR.

Summary of Changes
The following changes were made to the 2/26 revision of this document:
1. Updated Section 6.
2. Moved content into a new template with a new cover page and updated document fonts.
3. Made minor text edits.

Revised 2/26.

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