M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant Protocol
Instructions for Use of Product(s)
M3682, M3683, M3681
Literature # 9PIM368
Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus, Point Mutant (M-MLV RT (H–)) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5kb). The lack of RNase H activity is germane to the applications in that this activity can start to degrade templates and first-strand products when incubation times are long, as they may need to be when making long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option for the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
Printed in USA. Revised 10/16.