pGL4.15[luc2P/Hygro] Vector Protocol
Instructions for Use of Product(s)
E6701
Literature # 9PIE670
The pGL4.15[luc2P/Hygro] Vector encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.15[luc2P/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with a greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
Printed in USA. Revised 10/16.