Methylation Sensitivity of Isoschizomer Neoschizomer Pairs
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Isoschizomers and neoschizomers often differ in their sensitivity to methylation. This can be a simple way to differentiate the methylation state of DNA. Iso/neoschizomer pairs sold by Promega are listed below.
| Methylated Sequence | Cleaved by | Not Cleaved by |
|---|---|---|
| m4CCGG | MspI (C/CGG) | HpaII (C/CGG) |
| Cm5CGG | MspI (C/CGG) | HpaII (C/CGG) |
| Cm4CGG | MspI (C/CGG) | HpaII (C/CGG) |
| CCm5CGGG | XmaI (C/CCGGG) | SmaI (CCC/GGG) |
| Gm6ATC | Sau3AI (/GATC) | MboI, NdeII (/GATC) |
| GATm5C | MboI, NdeII (/GATC) | Sau3AI (/GATC) |
| GATm4C | MboI (/GATC) | Sau3AI (/GATC) |
| GGTACm5C | KpnI (GGTAC/C) | Acc65I (G/GTACC) |
Reference
McClelland, M., Nelson, M. and Raschke, E. (1994) Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases. Nucl. Acids Res. 22, 3640.