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Lys-C, Mass Spec Grade

Part Numbers: VA1170

VA1170_Lys-C-Mass-Spec-Grade_3
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Protease for Challenging Protein Digestions

  • Active under strong denaturing conditions
  • C-terminal (Lys-C) lysine cleavage
  • Generates longer peptides than with tryptic protein digestion

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Catalog number selected: VA1170

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Lys-C, Mass Spec Grade
20μg
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Active Under Strong Denaturing Conditions

Endoproteinase Lys-C, Mass Spec Grade, is a highly-purified serine protease that hydrolyzes specifically at the carboxyl side of lysines. Lys-C retains proteolytic activity under strong protein denaturing conditions such as 8M urea, which can be used to improve digestion of proteolytically resistant proteins. Lys-C, Mass Spec Grade, has optimal activity in the range of pH 7.0—9.0. This protease can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching.

Digestion Under Strong Denaturing Conditions

Lys-C or Lys-N protein digestion under strong denaturing conditions.
Panel A. One-step protein digestion under strong denaturing conditions. Panel B. Two-step protein digestion using strong denaturing conditions for the first step and diluting for trypsin digestion in the second step.

Native Lys-C Interchangeable with Recombinant Lys-C

Comparison of recombinant and native Lys-C protein digestions.

Comparison of lysine-specific cleavages. Mass Spec-Compatible Yeast Protein Extract, Intact (Cat.# V7341) was digested with either rLys-C (P. aeruginosa; Cat.# V1671) or native Lys-C (Lysobacter sp.; Cat.# VA1170) at a ratio of 1:100.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size

Lys-C Mass Spec Grade

 VA117A 1 × 20μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Similar Products

rChymoSelect™ MS Grade Protease Kit

Recombinant chymotrypsin engineered for precise, reproducible digests in peptide mapping workflows.

CS333204, CS333205

Arg-C Ultra, Mass Spec Grade

Endopeptidase that cleaves at the C-terminus of arginine residues, including Arg-Pro.

VA1831, VA1832

rAsp-N, Mass Spec Grade

Cleavage site: N-terminal of Asp. Optimal pH: 6–9.

VA1160

Glu-C, Sequencing Grade

Cleavage site: C-terminal of Glu. Optimal pH: 4–9.

V1651

Chymotrypsin, Sequencing Grade

Cleavage sites: C-terminal of Tyr, Phe, Trp. Optimal pH: 7–9.

V1061, V1062

Frequently Used With

IdeS and IdeZ Proteases

IgG-degrading enzymes that cleave at a single site below the hinge region.

V7511, V7515, V8341, V8345

Magnetic Proteomics Sample Prep (MPSP) Kit

Magnetic beads for streamlined, SP3-based proteomics sample preparation.

CS3325A04

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