PowerSeq® Quant MS System
Quantification of MiSeq® Platform Compatible MPS Libraries
- Enables normalization of MPS libraries based on DNA quantification
- Uses BRYT Green® dye-based qPCR system for maximum sensitivity and reproducibility
- Enables accurate and balanced multiplexed Illumina pooled libraries
Catalog Number:
Size
Catalog Number: PS5000
Consistent Quantification for MPS Libraries
The PowerSeq® Quant MS System is a qPCR-based system designed for library quantification within the MPS workflow by directly measuring the number of DNA molecules present in your libraries. Accurate quantification of a library determines the optimal amount of adapter ligated fragments for cluster generation and ensures even representation of each library on the chip.
Balanced Representation of Pooled Libraries
Use of the PowerSeq® Quant MS System ensures that your libraries are evenly represented and precisely quantified on the MiSeq® flowcell. This results in more consistent and reproducible sequencing results.
Thirty-two libraries were quantified using the PowerSeq® Quant MS System. The resulting values were used to normalize loading of samples into a final sequencing pool. The percent of reads identified for each sample from the total reads generated in the sequencing run demonstrates how evenly each sample was represented on the MiSeq® flowcell in relation to all libraries sequenced.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
PowerSeq® Quant 2X Master Mix |
DP115A | 5 × 1ml | ||
PowerSeq® Quant MS 10X Primer Mix |
DK803A | 1 × 1ml | ||
PowerSeq® Quant MS DNA Standard |
DD104A | 1 × 200μl | 200pM | |
PowerSeq® Quant MS 1X Dilution Buffer |
DM146A | 2 × 150ml | ||
|
Water, Amplification Grade |
DW099A | 2 × 1,250μl | View Product |
SDS
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Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.
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