AKT3 Kinase Enzyme System
Easily Screen and Profile AKT3 Kinase Inhibitors
- Includes kinase, substrate and reaction buffer
- Use with ADP-Gloâ„¢ Assay for bioluminescent detection of kinase activity
Catalog Number:
Size
Additional options
Catalog Number: V4010
Catalog Number: V6271
Catalog Number: V4011
Convenient, Scalable Kinase Profiling
The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The AKT3 Kinase Enzyme System contains:
- AKT3 Kinase, 10μg (Human, recombinant full-length). MW: ~85kDa.
- AKT (SGK) Peptide Substrate (RPRAATF); derived from the N-terminus of GSK3.
- Reaction Buffer, DTT.
Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT3 or Protein Kinase B γ (PKBγ) is a serine/threonine kinase that is a member of the AKT family. AKT3 is activated in cells exposed to stimuli such as hormones, growth factors and extracellular matrix components. AKT3 phosphorylates and regulates the function of many cellular proteins involved in processes that include cellular metabolism, survival/apoptosis and proliferation.
The AKT3 Kinase Enzyme System can be purchased with or without the ADP-Gloâ„¢ Kinase Assay reagents. Used together, the ADP-Gloâ„¢ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Gloâ„¢ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
See all Kinase Enzyme Systems available from Promega.
Protocols
Complete Protocol
Specifications
Catalog Number:
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size |
|---|---|---|
| AKT3 Kinase Enzyme System | V4010 | 1 × 10μg |
|
ADP-Gloâ„¢ Kinase Assay |
V9101 | 1 × 1,000 assays |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.U.S. Pat. No. 7,700,310 and other patents and patents pending.
U.S. Pat. No. 8,183,007 and other patents and patents pending.
Resources
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