Promega Corporation

Automated Methods

Sample Type	Instrument (click for method downloads)	Sample Volume (or amount)	Method Time (minutes)	Kit	Chemistry Type
Blood	Beckman Coulter Biomek® FX	200µl anticoagulated whole human blood	105	MagneSil® Genomic, Max Yield	Magnetic Resin-Based	More » Close Window TB: TB312 EP: N/A Output: 4-9µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated 96-Well Purification of Genomic DNA from Whole Blood
Blood	Tecan Freedom EVO®	1-10ml whole human blood	32 samples in 4 hours	ReliaPrep™ Large Volume HT gDNA System	Magnetic Resin-Based	More » Close Window TB: TM341 EP: N/A Output: ≥300µg human genomic DNA from 10ml sample; yield is variable based on WBC count and sample volume processed. Automation Company Website: Tecan
Blood	Beckman Coulter Biomek® FX	≤350µl anticoagulated whole human blood	150	ReliaPrep™ 96 gDNA Miniprep HT System	Magnetic Resin-Based	More » Close Window TB: TM368 EP: N/A Output: Up to 12µg human genomic DNA Automation Company Website: Beckman Coulter
Cultured Cells	Beckman Coulter Biomek® 2000	<1x10⁵ cultured cells	60	MagneSil® RNA	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Cultured Cells	Beckman Coulter Biomek® 3000	<1x10⁵ cultured cells	60	MagneSil® RNA	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Cultured Cells	Beckman Coulter Biomek® FX	<1x10³ cultured cells	100	MagneSil® RNA, 384 Well	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 50ng RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Cultured Cells	Beckman Coulter Biomek® FX	<1x10⁵ cultured cells	30	MagneSil® RNA, 96 Well	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Cultured Cells	Beckman Coulter Biomek® 2000	≤5x10⁶ cultured cells	45	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP018 Output: 5-10µg of genomic DNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Cultured Cells	Beckman Coulter Biomek® 3000	≤5x10⁶ cultured cells	35	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP018 Output: 5-10µg of genomic DNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Cultured Cells	Perkin Elmer Janus®	≤5x10⁶ cultured cells	45	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP018 Output: 5-10µg of genomic DNA from 1x10⁶ cells Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Cultured Cells	Beckman Coulter Biomek® FX	≤5x10⁶ cultured cells	55	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP018 Output: 5-10µg of genomic DNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Cultured Cells	Beckman Coulter Biomek® 2000	≤5x10⁶ cultured cells	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Cultured Cells	Beckman Coulter Biomek® 3000	≤5x10⁶ cultured cells	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Cultured Cells	Beckman Coulter Biomek® FX	≤5x10⁶ cultured cells	60	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Cultured Cells	Eppendorf epMotion® 5075 VAC	≤5x10⁶ cultured cells	90	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Collaborator Website with Methods: Eppendorf Automation Company Website: Eppendorf Article for the Chemistry: Perform Automated Nucleic Acid Purification Using the SV Systems
Cultured Cells	Perkin Elmer Janus®	≤5x10⁶ cultured cells	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Perkin Elmer Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Blood	Beckman Coulter Biomek® 3000	<20µl whole human blood	60	MagneSil® RNA	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Blood	Beckman Coulter Biomek® FX	60µl human whole blood	60	MagneSil® ONE Fixed Yield	Magnetic Resin-Based	More » Close Window TB: TB313 EP: N/A Output: 0.5-1.5µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated 96-Well Purification of Genomic DNA from Whole Blood
PCR Reaction	Beckman Coulter Biomek® 2000	≤100µl PCR Reaction	40	Wizard® SV 96 PCR Clean-Up	Vacuum Filtration-Based	More » Close Window TB: TB311 EP: EP008 Output: >70% recovery of fragments 100bp and larger, >90% recovery of fragments 300bp and larger Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV 96 PCR Clean-Up System
PCR Reaction	Eppendorf epMotion® 5075 VAC	≤100µl PCR Reaction	50	Wizard® SV 96 PCR Clean-Up	Vacuum Filtration-Based	More » Close Window TB: TB311 EP: EP008 Output: >70% recovery of fragments 100bp and larger, >90% recovery of fragments 300bp and larger Collaborator Website with Methods: Eppendorf Automation Company Website: Eppendorf Article for the Chemistry: Introducing the Wizard SV 96 PCR Clean-Up System
PCR Reaction	Perkin Elmer Janus®	≤100µl PCR Reaction	40	Wizard® SV 96 PCR Clean-Up	Vacuum Filtration-Based	More » Close Window TB: TB311 EP: EP008 Output: >70% recovery of fragments 100bp and larger, >90% recovery of fragments 300bp and larger Automation Company Website: Perkin Elmer Article for the Chemistry: Introducing the Wizard SV 96 PCR Clean-Up System
PCR Reaction	Beckman Coulter Biomek® FX	≤100µl PCR Reaction	20	Wizard® SV 96 PCR Clean-Up	Vacuum Filtration-Based	More » Close Window TB: TB311 EP: EP008 Output: >70% recovery of fragments 100bp and larger, >90% recovery of fragments 300bp and larger Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV 96 PCR Clean-Up System
Plant	Beckman Coulter Biomek® 2000	8mm leaf disc or 1-5 seeds	40	MagneSil® Plant	Magnetic Resin-Based	More » Close Window TB: TB289 EP: EP006 Output: 10ng-100ng gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated Genomic DNA Purification Using the Wizard Magnetic 96 DNA Plant System
Plant	Eppendorf epMotion® 5075 LH	8mm leaf disc or 1-5 seeds	60	MagneSil® Plant	Magnetic Resin-Based	More » Close Window TB: TB289 EP: EP006 Output: 10ng-100ng gDNA Automation Company Website: Eppendorf Article for the Chemistry: Automated Genomic DNA Purification Using the Wizard Magnetic 96 DNA Plant System
Plant	Beckman Coulter Biomek® FX	8mm leaf disc or 1-5 seeds	25	MagneSil® Plant	Magnetic Resin-Based	More » Close Window TB: TB289 EP: EP006 Output: 10ng-100ng gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated Genomic DNA Purification Using the Wizard Magnetic 96 DNA Plant System
Plant	Beckman Coulter Biomek® 3000	8mm leaf disc or 1-5 seeds	40	MagneSil® Plant	Magnetic Resin-Based	More » Close Window TB: TB289 EP: EP006 Output: 10ng-100ng gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated Genomic DNA Purification Using the Wizard Magnetic 96 DNA Plant System
Plant	Beckman Coulter Biomek® FX	1-2 leaf punches (6-8mm) or 1-2 seeds	40	MagneSil® Plant, Fixed Yield	Magnetic Resin-Based	More » Close Window TB: N/A EP: EP022 Output: 0.2-1.5µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Expanding the Capabilities of Plant Genomic DNA Purification
Plant	Beckman Coulter Biomek® FX	1-10 leaf punches (6-8mm) or 1-5 seeds	50	MagneSil® Plant, Inc Yield	Magnetic Resin-Based	More » Close Window TB: N/A EP: EP020 Output: 0.2-1.5µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Expanding the Capabilities of Plant Genomic DNA Purification
Plasmid	Beckman Coulter Biomek® FX	Bacterial culture ≤6 OD600	45	MagneSil® Plasmid	Magnetic Resin-Based	More » Close Window TB: TB286 EP: EP001 Output: 6-9µg of plasmid DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated Plasmid Purification Using MagneSil® Paramagnetic Particles
Plasmid	Beckman Coulter Biomek® 2000	Bacterial culture ≤6 OD600	75	MagneSil® Plasmid	Magnetic Resin-Based	More » Close Window TB: TB286 EP: EP001 Output: 6-9µg of plasmid DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated Plasmid Purification Using MagneSil® Paramagnetic Particles
Plasmid	Tecan Freedom EVO®	Bacterial culture ≤6 OD600	75	MagneSil® Plasmid	Magnetic Resin-Based	More » Close Window TB: TB286 EP: EP001 Output: 6-9µg of plasmid DNA Automation Company Website: Tecan Article for the Chemistry: Automated Plasmid Purification Using MagneSil® Paramagnetic Particles
Plasmid	Beckman Coulter Biomek® NX	bacterial culture ≤6 OD600	75	MagneSil® Plasmid	Magnetic Resin-Based	More » Close Window TB: TB286 EP: EP001 Output: 6-9µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Automated Plasmid Purification Using MagneSil® Paramagnetic Particles
Plasmid	Beckman Coulter Biomek® FX	bacterial culture ≤6 OD600	45	MagneSil® Plasmid Tfx	Magnetic Resin-Based	More » Close Window TB: TB314 EP: N/A Output: 6-20µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Wizard MagneSil® Tfx System for the Purification of Transfection-Grade DNA
Plasmid	Beckman Coulter Biomek® 2000	bacterial culture ≤6 OD600	75	MagneSil® Plasmid Tfx	Magnetic Resin-Based	More » Close Window TB: TB314 EP: N/A Output: 6-20µg gDNA Automation Company Website: Beckman Coulter Article for the Chemistry: Wizard MagneSil® Tfx System for the Purification of Transfection-Grade DNA
Plasmid	Beckman Coulter Biomek® FX	bacterial culture ≤4 OD600	60	Wizard® SV 96 Plasmid	Vacuum Filtration-Based	More » Close Window TB: TB272 EP: EP002 Output: Up to 13µg of plasmid DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Wizard SV 96 Plasmid DNA Purification System – High Quality Plasmid DNA
Plasmid	Beckman Coulter Biomek® 2000	bacterial culture ≤4 OD600	80	Wizard® SV 96 Plasmid	Vacuum Filtration-Based	More » Close Window TB: TB272 EP: EP002 Output: Up to 13µg of plasmid DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Wizard SV 96 Plasmid DNA Purification System – High Quality Plasmid DNA
Plasmid	Eppendorf epMotion® 5075 VAC	bacterial culture ≤4 OD600	90	Wizard® SV 96 Plasmid	Vacuum Filtration-Based	More » Close Window TB: TB272 EP: EP002 Output: Up to 13µg of plasmid DNA Collaborator Website with Methods: Eppendorf Automation Company Website: Eppendorf Article for the Chemistry: Wizard SV 96 Plasmid DNA Purification System – High Quality Plasmid DNA
Plasmid	Perkin Elmer Janus®	bacterial culture ≤4 OD600	80	Wizard® SV 96 Plasmid	Vacuum Filtration-Based	More » Close Window TB: TB272 EP: EP002 Output: Up to 13µg of plasmid DNA Automation Company Website: Perkin Elmer Article for the Chemistry: Wizard SV 96 Plasmid DNA Purification System – High Quality Plasmid DNA
Sanger Sequencing	Beckman Coulter Biomek® 2000	20µl sequencing reaction	45	MagneSil® Sequencing	Magnetic Resin-Based	More » Close Window TB: TB287 EP: EP021 Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp Automation Company Website: Beckman Coulter
Sanger Sequencing	Tecan Freedom EVO®	20µl sequencing reaction	45	MagneSil® Sequencing	Magnetic Resin-Based	More » Close Window TB: TB287 EP: EP021 Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp Automation Company Website: Tecan
Sanger Sequencing	Beckman Coulter Biomek® NX	20µl sequencing reaction	45	MagneSil® Sequencing	Magnetic Resin-Based	More » Close Window TB: TB287 EP: EP021 Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp Automation Company Website: Beckman Coulter
Sanger Sequencing	Beckman Coulter Biomek® FX	20µl sequencing reaction	25	MagneSil® Sequencing	Magnetic Resin-Based	More » Close Window TB: TB287 EP: EP021 Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp Automation Company Website: Beckman Coulter
Sanger Sequencing	Beckman Coulter Biomek® FX	10µl sequencing reaction	45	MagneSil® Sequencing, 384 well	Magnetic Resin-Based	More » Close Window TB: TB287 EP: EP010 Output: Readable sequence at 35bp past primer, >98% read accuracy out to 600bp Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput DNA Fragment Purification Using the MagneSil® Automated 384-Well Clean-Up Systems
Tissue	Perkin Elmer Janus®	≤5x10⁶ cultured cells	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Perkin Elmer Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Saliva	Beckman Coulter Biomek® FX	≤700µl Oragene saliva	150	ReliaPrep™ 96 gDNA Miniprep HT System	Magnetic Resin-Based	More » Close Window TB: TM368 EP: N/A Output: Up to 12µg human genomic DNA Automation Company Website: Beckman Coulter
Tissue	Beckman Coulter Biomek® 2000	≤20mg of tissue lysate; ≤1.2cm of mouse tail	50	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP007 Output: 5-20µg of genomic DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Tissue	Beckman Coulter Biomek® FX	≤20mg of tissue lysate; ≤1.2cm of mouse tail	40	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP007 Output: 5-20µg of genomic DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Tissue	Beckman Coulter Biomek® 3000	≤20mg of tissue lysate; ≤1.2cm of mouse tail	50	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP007 Output: 5-20µg of genomic DNA Automation Company Website: Beckman Coulter Article for the Chemistry: Introducing the Wizard SV and SV 96 Genomic DNA Purification Systems
Tissue	Eppendorf epMotion® 5075 VAC	≤20mg of tissue lysate; ≤1.2cm of mouse tail	60	Wizard® SV 96 Genomic	Vacuum Filtration-Based	More » Close Window TB: TB303 EP: EP007 Output: 5-20µg of genomic DNA Collaborator Website with Methods: Eppendorf Automation Company Website: Eppendorf Article for the Chemistry: Perform Automated Nucleic Acid Purification Using the SV Systems
Tissue	Beckman Coulter Biomek® 2000	≤30mg of tissue lysate	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Tissue	Beckman Coulter Biomek® 3000	≤30mg of tissue lysate	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Tissue	Perkin Elmer Janus®	≤30mg of tissue lysate	70	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Tissue	Beckman Coulter Biomek® FX	≤30mg of tissue lysate	60	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB294 EP: EP003 Output: Up to 5µg of Total RNA from 1x10⁶ cells Automation Company Website: Beckman Coulter Article for the Chemistry: High-Throughput Purification Using the SV 96 Total RNA Isolation System
Tissue	Eppendorf epMotion® 5075 VAC	≤30mg of tissue lysate	90	SV 96 Total RNA	Vacuum Filtration-Based	More » Close Window TB: TB312 EP: N/A Output: Up to 5µg of Total RNA from 1x10⁶ cells Collaborator Website with Methods: Eppendorf Automation Company Website: Eppendorf Article for the Chemistry: Perform Automated Nucleic Acid Purification Using the SV Systems
Tissue	Beckman Coulter Biomek® 2000	<2mg tissue lysate per well	60	MagneSil® RNA	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Tissue	Beckman Coulter Biomek® 3000	<2mg tissue lysate per well	60	MagneSil® RNA	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Tissue	Beckman Coulter Biomek® FX	<2mg tissue lysate per well	30	MagneSil® RNA, 96 Well	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Blood	Beckman Coulter Biomek® 2000	<20µl whole human blood	60	MagneSil® RNA	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Blood	Beckman Coulter Biomek® FX	<5µl whole human blood per well	100	MagneSil® RNA, 384 Well	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 50ng RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System
Blood	Beckman Coulter Biomek® FX	<20µl whole human blood	30	MagneSil® RNA, 96 Well	Magnetic Resin-Based	More » Close Window TB: TB328 EP: N/A Output: Up to 2.5µg RNA Automation Company Website: Beckman Coulter Article for the Chemistry: MagneSil® Total RNA Mini-Isolation System

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