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Unit 6: Basics of Molecular Cloning (Blue/White Selection)

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Other Units

Unit 1: The Relationship between Genes and Proteins
Unit 2 The Chemistry of Inheritance: DNA Structure and Replication
Unit 3: Detecting Microbial Contamination
Unit 4: Genomic DNA Purification
Unit 5: Solving a Crime Using DNA Analysis
Unit 7: Introduction to the Cell Cycle

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  • Unit Materials

This unit describes the methods and development of molecular cloning and blue/white selection. The laboratory is a math-intensive protocol that takes students from excising a gene fragment through subcloning, transformation, and gene expression.

Lecture Presentation

Basics of Molecular Cloning
(394 KB)

Laboratory Protocols

Instructor's Laboratory Manual
(105 KB)

Student's Laboratory Manual
(110 KB)

Related Cloning Resources

The Answer is There in Blue and White

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