Promega Corporation

Ultra-High-Throughput Profiling of Compounds Using Luminescent Assays Scientific Poster




Brad Larson1, Tracy Worzella1, and Mark Chong2
1Promega Corporation, Madison, WI, 2Aurora Discovery, San Diego, CA

Here we demonstrate the use of Promega luminescent, as well as fluorescent, HTS assays for profiling test compounds in a miniaturized ultra-high-throughput setting. Aurora Discovery’s BioRAPTR FRD™ Workstation was used to dispense cells, test compounds and assay reagents in a 1536-well format. IC50 calculations were performed for each compound and assay combination. Results show that IC50s from assays performed in a miniaturized uHTS setting can be used to create a clearer picture of the properties for individual compounds.

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  • Part# PS019
  • Printed in USA.

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