Renilla-Glo™ Luciferase Assay System Technical Manual

Literature # TM329

The Renilla-Glo™ Luciferase Assay System is a single-addition reagent that generates a glow-type signal with Renilla luciferase. When reconstituted, it has the capacity to lyse cells, reduce the autoluminescence of the coelenterazine substrate, and produce a stable signal (i.e., half-life greater than 60 minutes at 22°C). Renilla luciferase is about half the size of firefly (36kDa rather than 61kDa) and does not require ATP. The Renilla-Glo™ Luciferase Assay System offers a highly sensitive assay with high-signal stability.