Y Chromosome Deletion Detection System, Version 2.0 Technical Manual

Literature # TM248

The Y Chromosome Deletion Detection System, Version 2.0, provides a rapid method for the detection of specific regions of the human Y chromosome. This system is designed to detect deletions occurring in YqAZF. Further mapping of common deletion breakpoints relative to palindromes 1 through 8 may be performed in additional experiments. This system consists of 20 primer pairs that are homologous to previously identified and mapped sequence-tagged sites (STS). These primers will amplify nonpolymorphic short DNA segments from the Y chromosome when used in polymerase chain reactions (PCR). The primers have been combined into five sets for use in multiplex PCR. This makes it possible to determine the presence or absence of all 20 sequence-tagged sites by performing five parallel PCR amplifications.