The Maxwell® 16 Polyhistidine Protein Purification Kit allows simple, convenient, and robust purification of polyhistidine-tagged proteins from multiple sample types, including bacteria, mammalian cells, insect cells, and culture medium. The system also purifies HQ-tagged proteins from bacterial samples. The optimized automated method, compact instrumentation, and pre-filled reagent cartridges maximize performance while minimizing the hands-on time required for protein purification. The purified protein is compatible with many common downstream applications including polyacrylamide gel electrophoresis and detection, functionality studies, Western blot analysis, and mass spectrometry. In this article we demonstrate the performance of the Maxwell® 16 Polyhistidine Protein Purification Kit for low- to moderate-throughput automated protein purification. Purification results with the Maxwell® System are comparable to or better than those achieved using manual processing methods.
Promega Notes 95, 12–15.
Natalie Betz, Cristopher Cowan, Steve Krueger, Susan Fly, Jude Stevens and Michael Bjerke