Using Luciferase Reporters to Screen for GPCR Modulators

Previously, we described a strategy to construct stable cell lines expressing two luciferases on two plasmids for a GPCR reporter assay. In this report, we further demonstrate the utilities and advantages of the dual-reporter luciferase GPCR assay through additional examples including an agonist screen of a LOPAC compound collection.

Cell Notes 16, 22–25.