Promega Corporation

HaloTag® Ligand Building Blocks

The HaloTag® Ligand Building Blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications.

The HaloTag® Succinimidyl Ester (04) Ligand contains a reactive succinimidyl ester (SE) group connected to an alkyl chlo...

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HaloTag® Amine (O4) Ligand

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HaloTag® Amine (O4) Ligand

 Components

5mg
- P6741 € 550,00 Add to cart

HaloTag® Amine (O2) Ligand

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HaloTag® Amine (O2) Ligand

 Components

5mg
- P6711 € 550,00 Add to cart

HaloTag® Iodoacetamide (O4) Ligand

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HaloTag® Iodoacetamide (O4) Ligand

 Components

5mg
- P6771 € 550,00 Add to cart

HaloTag® Iodoacetamide (O2) Ligand

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HaloTag® Iodoacetamide (O2) Ligand

 Components

5mg
- P1681 Please Enquire

HaloTag® Succinimidyl Ester (O4) Ligand

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HaloTag® Succinimidyl Ester (O4) Ligand

 Components

5mg
- P6751 € 550,00 Add to cart

HaloTag® Succinimidyl Ester (O2) Ligand

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HaloTag® Succinimidyl Ester (O2) Ligand

 Components

5mg
- P1691 Please Enquire

HaloTag® Thiol (O4) Ligand

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HaloTag® Thiol (O4) Ligand

 Components

5mg
- P6761 € 550,00 Add to cart


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Storage Conditions

Store Cat.# P1691 and P6751 at or below –70°C under inert atmosphere. Store Cat.# P6711 and P6741 at or below –20°C in an air-tight container in the absence of light. Store Cat.# P1681, P6771 and P6761 at or below –20°C under inert atmosphere in the absence of light. See Promega Product Information for additional details on individual products.

For product intended use please see Patents & Disclaimers tab.

Example reaction with HaloTag Amine (O4) Ligand.Figure 1. Example reaction with HaloTag® Amine (O4) Ligand.

The HaloTag® Amine Ligand reacts with reporter groups containing activated carboxylic acid moieties through the amino end to form amide bonds. In this example, the amine is reacted with a succinimidyl ester. The conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

The HaloTag® Amine Ligand reacts with reporter groups containing activated carboxylic acid moieties through the amino end to form amide bonds. In this example, the amine is reacted with a succinimidyl ester. The conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

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Example reaction with HaloTag Amine (O2) Ligand.Figure 2. Example reaction with HaloTag® Amine (O2) Ligand.

The HaloTag® Amine Ligand reacts with reporter groups containing activated carboxylic acid moieties through the amino end to form amide bonds. In this example, the amine is reacted with a succinimidyl ester. The conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

The HaloTag® Amine Ligand reacts with reporter groups containing activated carboxylic acid moieties through the amino end to form amide bonds. In this example, the amine is reacted with a succinimidyl ester. The conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

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Example reaction with HaloTag Iodoacetamide (O4) Ligand.Figure 3. Example reaction with HaloTag® Iodoacetamide (O4) Ligand.

The HaloTag® Iodoacetamide Ligand reacts with sulfhydryl groups through the iodoacetamide end to form thioether bonds. The sulfhydryl-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

The HaloTag® Iodoacetamide Ligand reacts with sulfhydryl groups through the iodoacetamide end to form thioether bonds. The sulfhydryl-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

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Example reaction with HaloTag Iodoacetamide (O2) Ligand.Figure 4. Example reaction with HaloTag® Iodoacetamide (O2) Ligand.

The HaloTag® Iodoacetamide Ligand reacts with sulfhydryl groups through the iodoacetamide end to form thioether bonds. The sulfhydryl-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

The HaloTag® Iodoacetamide Ligand reacts with sulfhydryl groups through the iodoacetamide end to form thioether bonds. The sulfhydryl-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

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Example reaction with HaloTag Succinimidyl Ester (O4) Ligand.Figure 5. Example reaction with HaloTag® Succinimidyl Ester (O4) Ligand.

The HaloTag® Succinimidyl Ester Ligand reacts with an amine of a lysine amino acid residue through the succinimidyl ester end to form an amide bond. The amide-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

The HaloTag® Succinimidyl Ester Ligand reacts with an amine of a lysine amino acid residue through the succinimidyl ester end to form an amide bond. The amide-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

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Example reaction with HaloTag Succinimidyl Ester (O2) Ligand.Figure 6. Example reaction with HaloTag® Succinimidyl Ester (O2) Ligand.

The HaloTag® Succinimidyl Ester Ligand reacts with an amine of a lysine amino acid residue through the succinimidyl ester end to form an amide bond. The amide-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

The HaloTag® Succinimidyl Ester Ligand reacts with an amine of a lysine amino acid residue through the succinimidyl ester end to form an amide bond. The amide-conjugated ligand is then reacted with the HaloTag® protein through the alkylchloride functional group to covalently label the HaloTag® protein.

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Example reaction with HaloTag Thiol (O4) Ligand.Figure 7. Example reaction with HaloTag® Thiol (O4) Ligand.

The HaloTag® Thiol (O4) Ligand reacts with a functionalized (R group) iodoacetamide through the sulfhydryl end to form thioether bonds. In this example, the conjugated ligand is subsequently reacted with the HaloTag® protein through the alkyl chloride functional group to covalently label the HaloTag® protein.

The HaloTag® Thiol (O4) Ligand reacts with a functionalized (R group) iodoacetamide through the sulfhydryl end to form thioether bonds. In this example, the conjugated ligand is subsequently reacted with the HaloTag® protein through the alkyl chloride functional group to covalently label the HaloTag® protein.

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Use Restrictions

P6741, P6711, P6771, P1681, P6751, P1691, P6761 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

P6741, P6711, P6771, P1681, P6751, P1691, P6761 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may only use this product in their own research and may modify or link Promega HaloTag® ligands to Promega or customer-supplied moieties. No other use or transfer of this product is authorized without the express, written consent of Promega including, without limitation, commercial use. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

P6741, P6711, P6771, P1681, P6751, P1691, P6761 Patent Pending.

P6741, P6711, P6771, P1681, P6751, P1691, P6761 Certain applications of this product may require licenses from others.

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