Promega Corporation

TnT® Coupled Wheat Germ Extract System

The TnT® Coupled Wheat Germ Extract Systems offer researchers an alternative for eukaryotic cell-free protein expression: a one-tube, coupled transcription/translation system. The TnT® Extract Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard wheat germ extract translations commonly use RNA synthesized in vitro from SP6 or T7 RNA polymerase promoters. This entire process requires separate reactions with several steps between each reaction. The TnT® Extracts bypass many of these steps by incorporating transcription directly in the translation ...

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TnT® SP6 Coupled Wheat Germ Extract System

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TnT® SP6 Coupled Wheat Germ Extract System

 Components

  • TnT® Wheat Germ Extract

    L411A5 x 200μl
  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • TnT® SP6 Wheat Germ Polymerase

    L515A1 x 60μl
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
40 reactions
- L4130 € 720,00 Add to cart

TnT® T7 Coupled Wheat Germ Extract System

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TnT® T7 Coupled Wheat Germ Extract System

 Components

  • TnT® Wheat Germ Extract

    L411A5 x 200μl
  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • TnT® T7 Wheat Germ Polymerase

    L516A1 x 60μl
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
  • T7 Linear Control DNA

    P141A1 x 10μl
40 reactions
- L4140 € 720,00 Add to cart

TnT® T7/SP6 Coupled Wheat Germ Extract System

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TnT® T7/SP6 Coupled Wheat Germ Extract System

 Components

  • TnT® Wheat Germ Extract

    L411A5 x 200μl
  • Amino Acid Mixture Minus Cysteine

    L447C1 x 50μl
  • Luciferase Assay Wells

    L457A1 x 3 each
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • Luciferase SP6 Control DNA

    L474A1 x 5μg
  • Luciferase Assay Reagent

    L486A1 x 250μl
  • TnT® SP6 Wheat Germ Polymerase

    L515B1 x 30μl
  • TnT® T7 Wheat Germ Polymerase

    L516B1 x 30μl
  • Amino Acid Mixture Minus Leucine

    L995A1 x 50μl
  • Amino Acid Mixture Minus Methionine

    L996A1 x 50μl
  • T7 Linear Control DNA

    P141A1 x 10μl
40 reactions
- L5030 € 779,00 Add to cart


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Storage Conditions

Store the polymerase at –20°C. Store the Luciferase Assay Wells at room temperature. Store the other components at –70°C. Avoid multiple freeze-thaw cycles.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

L4130, L4140, L5030 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L4130, L4140, L5030 U.S. Pat. Nos. 5,641,641 and 5,650,289.

L4130, L4140, L5030 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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