Promega Corporation

T7 Sample System

The T7 Sample System is designed to facilitate the optimization of individual gene expression by offering four unique in vitro translation systems to evaluate. The system consists of samples of: TnT® T7 Quick for PCR DNA, TnT® T7 Quick Coupled Transcription/Translation System, TnT® Coupled Wheat Germ Extract System and E. coli T7 S30 Extract System for Circular DNA.

All of the coupled systems utilize RNA generated by a T7 phage promoter. Criteria such as post-translational modifications, ionic optimization and detection methods (i.e., non-isotopic) should be considered when choosing an in vitro system. In some ...

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T7 Sample System

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T7 Sample System

 Components

  • T7/S30 Extract for Circular DNA

    L114A1 x 150μl
  • Methionine

    L118A1 x 50μl
  • TnT® Wheat Germ Extract

    L411A1 x 200μl
  • Amino Acid Mixture, Complete

    L446A1 x 175μl
  • TnT® Reaction Buffer

    L462A1 x 90μl
  • Luciferase T7 Control DNA

    L482A1 x 5μg
  • S30 Premix without Amino Acids

    L512A1 x 750μl
  • TnT® T7 Wheat Germ Polymerase

    L516A1 x 60μl
  • TnT® T7 Quick Master Mix

    L520A1 x 200μl
  • TnT® T7 PCR Quick Master Mix

    L554A1 x 200μl
  • Amino Acid Mixture Minus Methionine

    L996B1 x 175μl
  • Nuclease-Free Water

    P119A1 x 1,250μl
1 each
- L5900 € 360,00 Add to cart


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Storage Conditions

Store at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

L5900 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L5900 U.S. Pat. Nos. 5,641,641 and 5,650,289.

L5900 Any use of the product for diagnostics requiring clearance or approval by the U.S. Food and Drug Administration or a foreign equivalent may require a license under Mayo Clinic U.S. Pat. No. 6,027,913.

L5900 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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