Promega Corporation

QuantiLum® Recombinant Luciferase

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QuantiLum® Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis) that provides the reliability and dependability needed for performing research or producing kits using bioluminescence reagents to detect ATP or luciferin substrates. A recombinant source eliminates the possibility of seasonal and regional variability that may be found in luciferase purified from natural sources.

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QuantiLum® Recombinant Luciferase

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QuantiLum® Recombinant Luciferase

 Components

  • rLuciferase

    E170A1 x 1mg
1mg
10–15mg/ml E1701 € 107,00 Add to cart

QuantiLum® Recombinant Luciferase

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QuantiLum® Recombinant Luciferase

 Components

  • rLuciferase

    E170B1 x 5mg
5mg
10–15mg/ml E1702 € 400,00 Add to cart


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QC Tests

  • Specific Activity: ≥2.0 × 1010 light units/mg protein.
  • SDS Page/Purity: >95%.

Source

An E. coli strain that carries a plasmid for the cloned luciferase gene from the North American firefly Photinus pyralis.

Storage Buffer

25mM Tris-acetate (pH 7.8 at 4°C), 1mM EDTA, 1mM DTT, 0.2M ammonium sulfate, 15% glycerol, 30% ethylene glycol.

Storage Conditions

Store at –70°C. Avoid multiple freeze-thaw cycles.

Unit Definition

The light unit value is the light output generated at room temperature over a 10-second period. QuantiLum® Recombinant Luciferase is diluted 106-fold in 1X Cell Culture Lysis Reagent (CCLR, Cat.# E1531) and 1mg/ml acetylated BSA. A quantity of 10μl of diluted luciferase is combined with 100μl of Luciferase Assay Reagent [prepared from Luciferase Assay Substrate and Luciferase Assay Buffer (supplied in Cat.# E1500)]. Light output is measured using a Turner Luminometer Model# TD-20e.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

E1701, E1702 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E1701, E1702 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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