Promega Corporation

Calpain-Glo™ Protease Assay

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities. Calpains are a family of calcium-activated cysteine proteases involved in cleaving a wide variety of proteins. Calpains modulate the biological activities of their substrates via limited proteolysis.

The Calpain-Glo™ Protease Assay provides a succinyl, proluminescent calpain substrate, Suc-LLVY-aminoluciferin, in a buffer system optimized for calpain and luciferase activities. The addition of the calpain reagent in an "add-mix-measure" format results in calpain cleavage of the substrate and ra...

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Calpain-Glo™ Protease Assay

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Calpain-Glo™ Protease Assay

 Components

  • Calpain-Glo™ Buffer

    G852A1 x 10ml
  • Suc-LLVY-Glo™ Substrate

    G856A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8501 € 408,00 Add to cart

Calpain-Glo™ Protease Assay

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Calpain-Glo™ Protease Assay

 Components

  • Calpain-Glo™ Buffer

    G852B1 x 50ml
  • Suc-LLVY-Glo™ Substrate

    G856B1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G8502 Please Enquire


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Storage Conditions

Store components at –20°C protected from light.

For product intended use please see Patents & Disclaimers tab.

Sensitivity of the Calpain-Glo Protease Assay compared to fluorescent assays.Figure 1. Sensitivity of the Calpain-Glo™ Protease Assay compared to fluorescent assays.

Use Restrictions

G8501, G8502 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8501, G8502 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G8501, G8502 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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